Difference between revisions of "Part:BBa K2382001:Design"

Latest revision as of 02:34, 30 October 2017

MSMEG_5998

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 428
1000
COMPATIBLE WITH RFC[1000]

Design Notes

It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1 The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)

Source

Mycobacterium smegmatis str. MC2 155 https://www.ncbi.nlm.nih.gov/nuccore/118467340/

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2382001 short</partinfo>
+===<span class='h3bb'>Sequence and Features</span>===
 <partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
@@ Line 7: / Line 8: @@
 ===Design Notes===
-It has not been coden optimized for E. Ccoli BL21, but it seems that there has no effect on protein expression.
+It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression.
+Otherwise, we remove the stop coden of NC_008596.1
+The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)
 ===Source===
+Mycobacterium smegmatis str. MC2 155
 https://www.ncbi.nlm.nih.gov/nuccore/118467340/
-===References===
-Lapalikar, G.V., et al., F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the Actinomycetales. PLoS One, 2012. 7(2): p. e30114.
-Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.