Difference between revisions of "Part:BBa I742142"

Latest revision as of 09:49, 7 October 2022

sam8 (tyrosine-ammonia lyase) coding sequence

This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)

iGEM2022-UCAS-China Contribution

We used sam8 in one of our plasmids for the production of ferulic acid from tyrosine: sam8-comt-sam5/pet-28a for vanillin synthesis BBa_K4256012.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 794
Illegal BamHI site found at 1196
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 537
Illegal AgeI site found at 484
Illegal AgeI site found at 653
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 88
Illegal BsaI site found at 354

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 __NOTOC__
 <partinfo>BBa_I742142 short</partinfo>
-This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)
+This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium ''Saccharothrix espanaensis'' DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete ''Saccharothrix espanaensis''' J. Bacteriol. 188 (7), 2666-2673 (2006)
+<p>
+<b><font size="4">iGEM2022-UCAS-China Contribution</font></b>
+</p>
+We used <i>sam8</i> in one of our plasmids for the production of ferulic acid from tyrosine: <i>sam8-comt-sam5</i>/pet-28a for vanillin synthesis <b>BBa_K4256012</b>.