Difference between revisions of "Part:BBa K2323005:Design"
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| − | + | This degradation tag is taken from Andersen et al., New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria, Applied and Environmental Microbiology, 64(6), June 1998. It can be degraded at a rate of 0.0095/min by the ClpP machinery of E.coli. | |
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===References=== | ===References=== | ||
| + | Andersen et al., New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria, Applied and Environmental Microbiology, 64(6), June 1998 | ||
Latest revision as of 13:39, 30 October 2017
GFP-ASV
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724
Design Notes
Source
This degradation tag is taken from Andersen et al., New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria, Applied and Environmental Microbiology, 64(6), June 1998. It can be degraded at a rate of 0.0095/min by the ClpP machinery of E.coli.
References
Andersen et al., New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria, Applied and Environmental Microbiology, 64(6), June 1998