Difference between revisions of "Part:BBa K2446055:Design"

 
(→‎Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This is the core part of the SynNotch. With synthetic extracellular recognition domain (SynECD, e.g. scFv),and the synthetic intracellular transcriptional domain (SynICD, e.g. SynTF), the other two parts of SynNotch which iare changeable by needs. The SynECD and SynICD can assembly with Notch core by fusion protein technique.
+
This is the core part of the SynNotch. With synthetic extracellular recognition domain (SynECD, e.g. scFv),and the synthetic intracellular transcriptional domain (SynICD, e.g. SynTF), the other two parts of SynNotch which are changeable by needs. The SynECD and SynICD can assembly with Notch core by fusion protein technique.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
RNA was extracted from mouse whole kidney, and cDNA was produced by commercial kit following corresponding protocol, and used as the template of PCR.
+
Fused by LaG16 BBa_K2446058, Mouse Notch core BBa_K2446056, tTA advance BBa_K2446057 from N terminal to C terminal.
  
 
===References===
 
===References===
 +
[1] L. Morsut et al., Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016).

Latest revision as of 19:34, 1 November 2017


SynNotch-anti-EGFP-tTA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2691
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1606


Design Notes

This is the core part of the SynNotch. With synthetic extracellular recognition domain (SynECD, e.g. scFv),and the synthetic intracellular transcriptional domain (SynICD, e.g. SynTF), the other two parts of SynNotch which are changeable by needs. The SynECD and SynICD can assembly with Notch core by fusion protein technique.

Source

Fused by LaG16 BBa_K2446058, Mouse Notch core BBa_K2446056, tTA advance BBa_K2446057 from N terminal to C terminal.

References

[1] L. Morsut et al., Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016).