Difference between revisions of "Part:BBa K2368020"
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<h1>Introduction</h1> | <h1>Introduction</h1> | ||
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<p> This part is the overlap of that sweetness receptor T1R3 combines with the BCP tag. </p> | <p> This part is the overlap of that sweetness receptor T1R3 combines with the BCP tag. </p> | ||
<p> The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and BCP tag.</P> | <p> The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and BCP tag.</P> | ||
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<p> Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.</p> | <p> Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.</p> | ||
[[File:T-BIT-China-2017parts-30.png|center|500px|默认文字]] | [[File:T-BIT-China-2017parts-30.png|center|500px|默认文字]] | ||
| − | <p style="text-align: center">Fig.1 The schematic diagram of BCP+T1R3 overlap</p> | + | <p style="text-align: center">Fig. 1 The schematic diagram of BCP+T1R3 overlap</p> |
[[File:T-BIT-China-2017parts-31.png|center|500px|默认文字]] | [[File:T-BIT-China-2017parts-31.png|center|500px|默认文字]] | ||
<p style="text-align: center">Fig. 2 The PCR method of BCP+T1R3 overlap</p> | <p style="text-align: center">Fig. 2 The PCR method of BCP+T1R3 overlap</p> | ||
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<p>This part is used to detect the expression and the location of the T1R3.</p> | <p>This part is used to detect the expression and the location of the T1R3.</p> | ||
[[File:T-BIT-China-2017parts-32.png|center|500px|默认文字]] | [[File:T-BIT-China-2017parts-32.png|center|500px|默认文字]] | ||
| − | <p style="text-align: center">Fig.3 Electrophoresis of BCP+T1R3 overlap.Line two is the target. </p> | + | <p style="text-align: center">Fig. 3 Electrophoresis of BCP+T1R3 overlap.Line two is the target. </p> |
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<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K2368020 parameters</partinfo> |
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Latest revision as of 16:03, 28 October 2017
Introduction
BCP+T1R3 overlap
This part is the overlap of that sweetness receptor T1R3 combines with the BCP tag.
The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and BCP tag.
The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.
Design
Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.
Fig. 1 The schematic diagram of BCP+T1R3 overlap
Fig. 2 The PCR method of BCP+T1R3 overlap
Experiment
This part is used to detect the expression and the location of the T1R3.
Fig. 3 Electrophoresis of BCP+T1R3 overlap.Line two is the target.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]