Difference between revisions of "Part:BBa I714070:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_I714070===
 
===Applications of BBa_I714070===
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The leakage of this part is almost zero.
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* The "Lock" part of riboswitch 3, help to block the expression of the gene downstream.
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The expression efficiency of this part can be enhanced to about 20%~30% when exposed to the RNA prodoct of <J23066>
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===User Reviews===
 
===User Reviews===
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<partinfo>BBa_I714070 AddReview number</partinfo>
 
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<I>Username</I>
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<I>nianhui</I>
 
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Enter the review inofrmation here.
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This part couldn't be transformed and had bad sequencing results, inconsistent resistance,and bad gel results.We re-constructed this part by assembling two basic parts Ptet (BBa_R0040) and [lock3d] (BBa_J23032).Sequencing results suggested it was successfully assembled. A lot of quantitative results also validate its function. See BBa_K1087004
 
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*data can be found http://2007.igem.org/Peking_Hop-Count
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2013 SCU_China.  http://2013.igem.org/Team:SCU_China/Project  This part BBa_I714070 couldn't be transformed because of its bad sequencing results, inconsistent resistance,and bad gel results.We re-constructed this part by assembling two basic parts Ptet (BBa_R0040) and [lock3d] (BBa_J23032).Sequencing results suggested it was successfully assembled. A lot of quantitative results also validate its function. See BBa_K1087004]

Latest revision as of 02:44, 28 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I714070

  • The "Lock" part of riboswitch 3, help to block the expression of the gene downstream.

User Reviews

UNIQ89d314f257827cef-partinfo-00000000-QINU UNIQ89d314f257827cef-partinfo-00000001-QINU

  • data can be found http://2007.igem.org/Peking_Hop-Count

2013 SCU_China. http://2013.igem.org/Team:SCU_China/Project This part BBa_I714070 couldn't be transformed because of its bad sequencing results, inconsistent resistance,and bad gel results.We re-constructed this part by assembling two basic parts Ptet (BBa_R0040) and [lock3d] (BBa_J23032).Sequencing results suggested it was successfully assembled. A lot of quantitative results also validate its function. See BBa_K1087004]