Difference between revisions of "Part:BBa K2230018"
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=== Research === | === Research === | ||
| − | Based on our research, the glucose transporter of ''Salmonella'' has a lower Km compared to human small intestine, ''Staphylococcus'' and ''E. coli'', indicating a higher efficiency for glucose uptake. | + | Based on our research, the glucose transporter of ''Salmonella'' has a lower Km compared to human small intestine, ''Staphylococcus'' and ''E. coli'', indicating a higher efficiency for glucose uptake. In our study, we demonstrated glucose absorption ability by overexpressing each of these two systems from ''Salmonella'' in ''E. coli''. Please go to our wiki page ([http://2017.igem.org/Team:Mingdao/Demonstrate Mingdao iGEM 2017]) for more information. |
[[File:Mingdaophil1026-2.png|550px|center]] | [[File:Mingdaophil1026-2.png|550px|center]] | ||
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=== Cloning === | === Cloning === | ||
| − | The crr gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3 | + | The crr gene was amplified from gDNA of ''Salmonella typhimurium'' and cloned onto pSB1C3 |
[[File:Mingdaophil1026-3.jpeg|400px|center]] | [[File:Mingdaophil1026-3.jpeg|400px|center]] | ||
Latest revision as of 05:50, 26 October 2017
crr/pSB1C3
Salmonella typhimurium LT2 has two glucose-specific transporter systems, PTS system and sodium/glucose cotransporter. PTS system contains two subunits IIA encoded by crr and IIBC by ptsG which are assembled to a high-affinity active transporter. The other is a Na+/glucose cotransporter encoded by STM1128 that contributes to facilitated transport with lower glucose affinity.
Research
Based on our research, the glucose transporter of Salmonella has a lower Km compared to human small intestine, Staphylococcus and E. coli, indicating a higher efficiency for glucose uptake. In our study, we demonstrated glucose absorption ability by overexpressing each of these two systems from Salmonella in E. coli. Please go to our wiki page ([http://2017.igem.org/Team:Mingdao/Demonstrate Mingdao iGEM 2017]) for more information.
Cloning
The crr gene was amplified from gDNA of Salmonella typhimurium and cloned onto pSB1C3
Reference
1. Glucose Galactose Malabsorption. American Journal of Physiology - Gastrointestinal and Liver Physiology 1998;275:G879-G882
2. Functional Properties and Genomics of Glucose Transporters. Curr Genomics. 2007;8(2): 113–128.
3. The SLC2 (GLUT) Family of Membrane Transporters. Mol Aspects Med. 2013;34(0): 121–138.
4. Glucose and Glycolysis Are Required for the Successful Infection of Macrophages and Mice by Salmonella enterica Serovar Typhimurium. Infect Immun. 2009;77(7): 3117–3126.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 166
- 1000COMPATIBLE WITH RFC[1000]