Difference between revisions of "Part:BBa K2520013"

 
 
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<partinfo>BBa_K2520013 short</partinfo>
 
<partinfo>BBa_K2520013 short</partinfo>
  
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This device is a complete system for displaying proteins on cells' membrane. The IgK leader (<partinfo>K2520024</partinfo>) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (<partinfo>K2520026</partinfo>) is a trans-membrane domain that anchors all the components located between the IgK leader and the PDGFR itself to the membrane. HA (<partinfo>K2520030</partinfo>) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of multiple sclerosis (<partinfo>K2520038</partinfo>) and is component related to our specific project.
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The CMV promoter (<partinfo>K1119006</partinfo>) is a constitutive expression promoter in mammalian cells and hGH (<partinfo>K404108</partinfo>) serves as a terminator.
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===MS disease===
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In MS, the immune system attacks the protective sheath (myelin) that covers nerve fibers and causes communication problems between your brain and the rest of your body. Eventually, the disease can cause the nerves themselves to deteriorate or become permanently damaged.
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In MS disease, T cells attack 3 types of glycoprotein:
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1) Myelin basic protein (MBP)
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2) Proteolipid protein (PLP)
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3) myelin oligodendrocyte
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glycoprotein (MOG)
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[[File:ms.gif|400px|thumb|center|]]
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<u>Experimental  autoimmune encephalomyelitis (EAE)</u>:
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EAE is a CD4' T cell-mediated inflammatory disease of the central nervous  system  (CNS)  induced  in  experimental  animals with CNS homogenate, myelin or myelin components. In its  chronic  form,  EAE is a well  accepted  experimental model  for  multiple  sclerosis (MS).
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<u>Epitopes</u>:
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MOG glycoprotein is the only CNS autoantigen known to induce both a T-cell response and a demyelinating autoantibody response in EAE
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Therefore, we chose the three epitopes that induced the most significant immune response.
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MOG1 is the first epitope that appears in the MOG glycoprotein sequence (1-21)
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===References===
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Mendel, Itzhack, Nicole Kerlero de Rosbo, and Avraham Ben‐Nun. "A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H‐2b mice: Fine specificity and T cell receptor Vβ expression of encephalitogenic T cells." European journal of immunology 25.7 (1995): 1951-1959.‏
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APA
  
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===Usage and Biology===
 
  
 
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Latest revision as of 11:52, 31 October 2017


CMV-Mono display:MOG 1-hGH

This device is a complete system for displaying proteins on cells' membrane. The IgK leader (BBa_K2520024) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (BBa_K2520026) is a trans-membrane domain that anchors all the components located between the IgK leader and the PDGFR itself to the membrane. HA (BBa_K2520030) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of multiple sclerosis (BBa_K2520038) and is component related to our specific project. The CMV promoter (BBa_K1119006) is a constitutive expression promoter in mammalian cells and hGH (BBa_K404108) serves as a terminator.

MS disease

In MS, the immune system attacks the protective sheath (myelin) that covers nerve fibers and causes communication problems between your brain and the rest of your body. Eventually, the disease can cause the nerves themselves to deteriorate or become permanently damaged.

In MS disease, T cells attack 3 types of glycoprotein:

1) Myelin basic protein (MBP)

2) Proteolipid protein (PLP)

3) myelin oligodendrocyte glycoprotein (MOG)

Ms.gif


Experimental autoimmune encephalomyelitis (EAE):

EAE is a CD4' T cell-mediated inflammatory disease of the central nervous system (CNS) induced in experimental animals with CNS homogenate, myelin or myelin components. In its chronic form, EAE is a well accepted experimental model for multiple sclerosis (MS).

Epitopes:

MOG glycoprotein is the only CNS autoantigen known to induce both a T-cell response and a demyelinating autoantibody response in EAE

Therefore, we chose the three epitopes that induced the most significant immune response. MOG1 is the first epitope that appears in the MOG glycoprotein sequence (1-21)

References

Mendel, Itzhack, Nicole Kerlero de Rosbo, and Avraham Ben‐Nun. "A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H‐2b mice: Fine specificity and T cell receptor Vβ expression of encephalitogenic T cells." European journal of immunology 25.7 (1995): 1951-1959.‏ APA


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1417