Difference between revisions of "Part:BBa K2429091:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The specific motif we’re targeting on the intron is the 3’ splice site. Therefore, the guide sequences tend to target the end of the intron. Furthermore, the guides share a lot of base pairs because they are essentially the same sequences but shifted a certain amount. This practice of tiling guides allows us to make more precise conclusions about what intronic sequences are important for splicing. | |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | The guide sequences were synthesized by IDT. | |
===References=== | ===References=== | ||
Latest revision as of 19:06, 25 October 2017
mKate FF4 Guide Family Member
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The specific motif we’re targeting on the intron is the 3’ splice site. Therefore, the guide sequences tend to target the end of the intron. Furthermore, the guides share a lot of base pairs because they are essentially the same sequences but shifted a certain amount. This practice of tiling guides allows us to make more precise conclusions about what intronic sequences are important for splicing.
Source
The guide sequences were synthesized by IDT.