Difference between revisions of "Part:BBa K2235009:Experience"

(→‎Molecular cloning)
 
Line 4: Line 4:
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
−
===Molecular cloning===
 
  
−
'''Ligation of sialidase composite insert into pSB1C3'''
 
−
 
−
The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
 
−
 
−
[[File:SiaComp_1.png|600px|thumb|left|Figure 1]]
 
−
 
−
From left to right: M DNA ladder, next 2 lanes are plasmids digested with Ecor1 and Pst1.
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 18:45, 25 October 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


User Reviews

UNIQb8e36b649d545657-partinfo-00000000-QINU UNIQb8e36b649d545657-partinfo-00000001-QINU