Difference between revisions of "Part:BBa K2235009:Experience"
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Molecular cloning===
 
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'''Ligation of sialidase composite insert into pSB1C3'''
 
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The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
 
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[[File:SiaComp_1.png|600px|thumb|left|Figure 1]]
 
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Latest revision as of 18:45, 25 October 2017


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