Difference between revisions of "Part:BBa K2368014"
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__NOTOC__ | __NOTOC__ | ||
<h1>Introduction</h1> | <h1>Introduction</h1> | ||
| − | <partinfo>BBa_K2368014 short</partinfo> | + | <p style="text-align: center"><partinfo>BBa_K2368014 short</partinfo></p> |
<p>This part is the overlap of that sweetness receptor T1R2 combines with the BCP tag. </p> | <p>This part is the overlap of that sweetness receptor T1R2 combines with the BCP tag. </p> | ||
<p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and BCP tag.</P> | <p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and BCP tag.</P> | ||
<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.</p> | <P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.</p> | ||
<h1>Design</h1> | <h1>Design</h1> | ||
| − | <p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and BCP. Then, we combined the overlap of | + | <p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and BCP. Then, we combined the overlap of T1R2 with the BCP by PCR.</p> |
[[File:T-BIT-China-2017parts-24.png|center|500px|默认文字]] | [[File:T-BIT-China-2017parts-24.png|center|500px|默认文字]] | ||
<p style="text-align: center">Fig.1 The schematic diagram of BCP+T1R2 overlap</p> | <p style="text-align: center">Fig.1 The schematic diagram of BCP+T1R2 overlap</p> | ||
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<p>This part is used to detect the expression and the location of the T1R2.</p> | <p>This part is used to detect the expression and the location of the T1R2.</p> | ||
[[File:T-BIT-China-2017parts-26.png|center|500px|默认文字]] | [[File:T-BIT-China-2017parts-26.png|center|500px|默认文字]] | ||
| − | <p style="text-align: center">Fig.3 Electrophoresis of BCP+T1R2 overlap. Line | + | <p style="text-align: center">Fig.3 Electrophoresis of BCP+T1R2 overlap. Line 3 and 4 is the target.</p> |
Latest revision as of 19:10, 26 October 2017
Introduction
T1R2-BCP
This part is the overlap of that sweetness receptor T1R2 combines with the BCP tag.
The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and BCP tag.
The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.
Design
Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and BCP. Then, we combined the overlap of T1R2 with the BCP by PCR.
Fig.1 The schematic diagram of BCP+T1R2 overlap
Fig. 2 The PCR method of BCP+T1R2 overlap
Experiment
This part is used to detect the expression and the location of the T1R2.
Fig.3 Electrophoresis of BCP+T1R2 overlap. Line 3 and 4 is the target.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]