Difference between revisions of "Part:BBa K2368013"
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__NOTOC__ | __NOTOC__ | ||
<h1>Introduction</h1> | <h1>Introduction</h1> | ||
| − | <partinfo>BBa_K2368013 short</partinfo> | + | <p style="text-align: center"><partinfo>BBa_K2368013 short</partinfo></P> |
| − | <p> This part is the overlap of that sweetness receptor T1R3 combines with the | + | <p> This part is the overlap of that sweetness receptor T1R3 combines with the YCP tag. </p> |
<p> The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and YCP tag.</P> | <p> The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and YCP tag.</P> | ||
<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.</p> | <P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.</p> | ||
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<p>This part is used to detect the expression and the location of the T1R3.</p> | <p>This part is used to detect the expression and the location of the T1R3.</p> | ||
[[File:T-BIT-China-2017parts-19.png|center|500px|默认文字]] | [[File:T-BIT-China-2017parts-19.png|center|500px|默认文字]] | ||
| − | <p style="text-align: center">Fig.3 Electrophoresis of YCP+T1R3 overlap. </p> | + | <p style="text-align: center">Fig.3 Electrophoresis of YCP+T1R3 overlap.Line 1 and 2 is the target. </p> |
Latest revision as of 19:09, 26 October 2017
Introduction
T1R3-YCP
This part is the overlap of that sweetness receptor T1R3 combines with the YCP tag.
The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and YCP tag.
The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.
Design
Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and YCP. Then, we combined the overlap of T1R3 with the YCP by PCR.
Fig.1 The schematic diagram of YCP+T1R3 overlap
Fig. 2 The PCR method of YCP+T1R3 overlap
Experiment
This part is used to detect the expression and the location of the T1R3.
Fig.3 Electrophoresis of YCP+T1R3 overlap.Line 1 and 2 is the target.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]