Difference between revisions of "Part:BBa K2267028"
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<partinfo>BBa_K2267028 short</partinfo> | <partinfo>BBa_K2267028 short</partinfo> | ||
+ | we Codon optimization Part:BBa_K319039 in G.xylinus | ||
− | + | we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are | |
+ | |||
+ | plux1: https://parts.igem.org/Part:BBa_K2267029 | ||
+ | |||
+ | plux3: https://parts.igem.org/Part:BBa_K2267030 | ||
+ | |||
+ | plux4: https://parts.igem.org/Part:BBa_K2267031 | ||
+ | |||
+ | plux6: https://parts.igem.org/Part:BBa_K2267032 | ||
+ | |||
+ | plux8: https://parts.igem.org/Part:BBa_K2267046 | ||
+ | |||
+ | listed below. | ||
+ | We linked gfp reporter genes | ||
+ | luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043 | ||
+ | |||
+ | luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041 | ||
+ | |||
+ | luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042 | ||
+ | |||
+ | luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044 | ||
+ | |||
+ | luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045 | ||
+ | |||
+ | on them respectively to test the function of modified promoters. | ||
+ | |||
+ | https://static.igem.org/mediawiki/2017/e/e6/T--TUST_China--part_part_results.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 04:00, 2 November 2017
GFP
we Codon optimization Part:BBa_K319039 in G.xylinus
we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are
plux1: https://parts.igem.org/Part:BBa_K2267029
plux3: https://parts.igem.org/Part:BBa_K2267030
plux4: https://parts.igem.org/Part:BBa_K2267031
plux6: https://parts.igem.org/Part:BBa_K2267032
plux8: https://parts.igem.org/Part:BBa_K2267046
listed below. We linked gfp reporter genes luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043
luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041
luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042
luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044
luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045
on them respectively to test the function of modified promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 664