|
|
| (5 intermediate revisions by one other user not shown) |
| Line 5: |
Line 5: |
| | | | |
| | ===Applications of BBa_K2368003=== | | ===Applications of BBa_K2368003=== |
| − | | + | Please see it at the main page. |
| − | In our experiment, this part is used to accept the signal inputted in our whole pathway.
| + | |
| − | <p>Part 1: Dry experiments of T1R2</p>
| + | |
| − | <p>1、Structure models</p>
| + | |
| − | <p>Purpose </p>
| + | |
| − | <p>In order to confirm whether this “radar” T1R2/T1R3 can “sense” the sweetness of different sweetener, we simulate the receptor structure model. It helps us to understand how the sweeteners binding to T1R2/T1R3 receptor visually. Otherwise we hope to find some unknown sweeteners binding sites based on this model, even some ideal but unknown sweeteners. </p>
| + | |
| − | <p>Method</p>
| + | |
| − | <p>Initially, to make the signal input more accurate and reliable, we simulate the T1R2/T1R3 receptor structure model using SWISS-MODEL. Additionally, we prepare some sweeteners’ PDB file based on Chemdraw 2D and Chemdraw 3D. And the docking process is performed by using Autodock Vina.</p>
| + | |
| − | <p>Result</p>
| + | |
| − | <p>We used homology modeling method to get the structure of human sweet receptorT1R2/T1R3. The quality of the simulate structure within normal range and it only contain the ligand-binding-domain based on the crystal protein structure of Mice.</p>
| + | |
| − | | + | |
| − | [[File:T-BIT-China-2017parts-22.png|center|250px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 2 The simulate structure of human sweetness receptors ligand-binding domain (LBD)</i></p>
| + | |
| − | | + | |
| − | <p>Then we use software Chemdraw 2D and Chemdraw 3D to build PDB files of some classic sweeteners.</p>
| + | |
| − | | + | |
| − | [[File:T-BIT-China-2017parts-33.png|center|500px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 3 The 3D structure of some sweeteners</i></p><p></p>
| + | |
| − | <p>The docking process is carried out under Autodock Vina (Fig. 4-6).</p>
| + | |
| − | [[File:T-BIT-China-2017parts-4.png|center|250px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 4 The docking result of different sweeteners </i></p>
| + | |
| − | [[File:T-BIT-China-2017parts-5.png|center|250px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 5 The docking result of aspartame </i></p>
| + | |
| − | [[File:T-BIT-China-2017part-6.png|center|250px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 6 The docking result of stevioside </i></p>
| + | |
| − | <p>2、T1R2/T1R3 receptor activation part </p>
| + | |
| − | <p>The part is about the sweeteners binding to the receptor and the GPCR produce the initial signal through changing structure. For T1R2/T1R3, we divide the process of inducing signal into four conditions of T1R2/T1R3 as show in Fig. 7. And the reaction between different conditions of protein is used ODEs to describe it. The all ODEs are shown follow. </p>
| + | |
| − | [[File:T-BIT-China-2017parts-7.png|center|500px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 7 T1R2/T1R3 receptor activation reaction diagram </i></p>
| + | |
| − | | + | |
| − | | + | |
| − | [[File:T-BIT-China-2017parts-8.png|center|500px|默认文字]]
| + | |
| − | <p>(T1R2/3: the T1R2/T1R3 heterodimer)</p>
| + | |
| − | <p>The parameters of this part are listed in the Table 1.</p>
| + | |
| − | [[File:T-BIT-China-2017parts-9.png|center|500px|默认文字]]
| + | |
| − | <p>The output of the T1R2/T1R3 receptor activation part is showed. (Fig.8)</p>
| + | |
| − | [[File:T-BIT-China-2017parts-10.png|center|500px|默认文字]]
| + | |
| − | <p style="text-align: center">Fig. 8 The output signal, activated state of T1R2/T1R3, induced by different concentration ligand </i></p>
| + | |
| − | <p></p>
| + | |
| − | <p></p>
| + | |
| − | <p></p>
| + | |
| − | <p></p>
| + | |
| − | <p></p>
| + | |
| − | <p></p>
| + | |
| | ===User Reviews=== | | ===User Reviews=== |
| | <!-- DON'T DELETE --><partinfo>BBa_K2368003 StartReviews</partinfo> | | <!-- DON'T DELETE --><partinfo>BBa_K2368003 StartReviews</partinfo> |
UNIQ7fa979f394eed927-partinfo-00000000-QINU
UNIQ7fa979f394eed927-partinfo-00000001-QINU
