Difference between revisions of "Part:BBa K2492002:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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Universal mCherry coding sequence of three introns for good expression in C.elegans.
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how you used this part and how it worked out.
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MCherry is a commonly used reporter. Since ordinary mCherry(part BBa_K2005050) may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately.
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The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).
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[1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254.
  
 
===Applications of BBa_K2492002===
 
===Applications of BBa_K2492002===
  
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[[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|600px|thumb|left|mcherry expressed in AWA]]
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[[File: T--SUSTech_Shenzhen--C.elegans_AWA_mcherry.png|500px|thumb|left|mcherry expressed in AWA]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 17:31, 1 November 2017


Universal mCherry coding sequence of three introns for good expression in C.elegans. MCherry is a commonly used reporter. Since ordinary mCherry(part BBa_K2005050) may not have high expression in C.elegans, artificial introns are inserted in the constructed plasmid[1]. We can obtain ord-10::Chrimson::GEM-GECO::mCherry transgenic offsprings microinjection. MCherry driven by promoter ord-10(Part:BBa_K2492004) indicates the position of neuron AWA. Moreover the fluorescence of mCherry aids the imaging for the neuron AWA pattern. The intensity of fluorescence reveals the expression level approximately. The mCherry part we used now is optimized for C.elegans and perform excellently observed under confocal microscope. We added the figure to part page The whole neuron with dendrite can be observed distinctly. The branch of dendrite near the mouse can be observed as well(Figure).

[1]:S.Redemann. (2011). codon adaptation– based control of protein expression in C. elegans, Nature Methods, Vol.8 No.3 250-254.

Applications of BBa_K2492002

mcherry expressed in AWA

User Reviews

UNIQa81ef0ec541c5f67-partinfo-00000000-QINU UNIQa81ef0ec541c5f67-partinfo-00000001-QINU