Difference between revisions of "Part:BBa K2368005"
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<h1>Introduction</h1> | <h1>Introduction</h1> | ||
| − | <partinfo>BBa_K2368005 short</partinfo> | + | <p style="text-align: center"><partinfo>BBa_K2368005 short</partinfo></p> |
| − | <P><i>P<sub>fus</sub></i> is an induced promoter from <i>Saccharomyces cerevisiae</i>. In the endogenous MAPK pathway in <i> | + | <P><i>P<sub>fus</sub></i> is an induced promoter from <i>Saccharomyces cerevisiae</i>. In the endogenous MAPK pathway in <i>Saccharomyces cerevisiae</i>, when Ste2 binding with α pheromone, Ste12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. </P> |
<P>However, the sequence of <i>P<sub>fus</sub></i> contains <i>Pst1</i> restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P> | <P>However, the sequence of <i>P<sub>fus</sub></i> contains <i>Pst1</i> restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P> | ||
<div id="section"> | <div id="section"> | ||
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[[File:T_BIT-China_2017part_K2368005.jpeg|center|300px|标题 ]] | [[File:T_BIT-China_2017part_K2368005.jpeg|center|300px|标题 ]] | ||
<p style="text-align: center">Fig.1 The sequencing result of <i>P<sub>fus</sub></i></p> | <p style="text-align: center">Fig.1 The sequencing result of <i>P<sub>fus</sub></i></p> | ||
| + | <p>This part is an improvement of [https://parts.igem.org/Part:BBa_K1072023 BBa_K1072023] of 2013 SCUT team.</p> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 18:04, 26 October 2017
Introduction
Pfus
Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in Saccharomyces cerevisiae, when Ste2 binding with α pheromone, Ste12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.
However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)
Fig.1 The sequencing result of Pfus
This part is an improvement of BBa_K1072023 of 2013 SCUT team.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]