Difference between revisions of "Part:BBa K2492000:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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We successfully improve characterization of parts BBa_K309016—GFP with introns used in C.elegans. What’s more, we optimize the sequence to make the coding sequence stats with “ATG”. The optimized GFP is submitted.
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how you used this part and how it worked out.
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We construct a str-1::Chrimson::GEMgeco::GFP fusion gene and inject it into C.elegans. The function of GFP is to localize the neuron AWB with the help of promoter str-1.
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We capture the image of GFP specifically expressed in AWB with confocal microscope and clearly observe AWB even its dendrite.
  
 
=== Applications of BBa_K2492000 ===
 
=== Applications of BBa_K2492000 ===
  
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[[File: T--SUSTech_Shenzhen--C.elegans_AWB_GFP.png]]
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[[File: T--SUSTech_Shenzhen--C.elegans_AWB_GFP.png|800px|thumb|left|GFP expressed in AWB|GFP expressed in AWB]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 12:57, 25 October 2017


We successfully improve characterization of parts BBa_K309016—GFP with introns used in C.elegans. What’s more, we optimize the sequence to make the coding sequence stats with “ATG”. The optimized GFP is submitted. We construct a str-1::Chrimson::GEMgeco::GFP fusion gene and inject it into C.elegans. The function of GFP is to localize the neuron AWB with the help of promoter str-1. We capture the image of GFP specifically expressed in AWB with confocal microscope and clearly observe AWB even its dendrite.

Applications of BBa_K2492000

GFP expressed in AWB

User Reviews

UNIQ8e8593c73788a0b5-partinfo-00000000-QINU UNIQ8e8593c73788a0b5-partinfo-00000001-QINU