Difference between revisions of "Part:BBa K2282004"
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Latest revision as of 12:47, 25 October 2017
Phage lambda promoter regulated by the cI857 repressor
Usage and Biology
Leftward promoter (pL) deriving from phage ƛ is fully repressed at low temperature by the thermolabile cI857 protein. cI857 loss repression activity on pL promoter when temperature rise above 30°C until 42°C when it can no longer bind to pL.
Design consideration
No particular design consideration is to be notified here.
References
Norma A Valdez-Cruz, Luis Caspeta, Néstor O Pérez, Octavio T Ramírez, Mauricio A Trujillo-Roldán - Production of recombinant proteins in E.coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters - Microbial Cell Factories 2010, 9:18.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]