Difference between revisions of "Part:BBa K2381010"

Latest revision as of 02:36, 2 November 2017

Cod1+ rigid linker+pMagFast

In this part, the light-induced dimerization protein,pMag[1], is fused with the C end of split dCas9 through rigid linker.
The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.
Its amion acid sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1099
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2277
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2381010 short</partinfo>
-In this part, the light-induced dimerization protein,<a href="https://parts.igem.org/Part:BBa_K2381001">pMag</a>, is fused with the N fragment of split dCas9 through rigid linker.<br/>
+<p>In this part, the light-induced dimerization protein,pMag[https://parts.igem.org/Part:BBa_K2381000], is fused with the C end of split dCas9 through rigid linker.<br/>
-The alpha-helix structure of the rigid linker render more cooperativity between split dCas9 and dimerization protein.<br/>
+The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.<br/>
-It's protein sequence is A(EAAAK)3A, the flanking Ala guarantees a flexibility conjunction, which will decrease the effect on the function of protein.
+Its amion acid sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein. </p>
 <!-- Add more about the biology of this part here