Difference between revisions of "Part:BBa K2486021"

Latest revision as of 21:59, 1 November 2017

PdtxR/DtxR based Fe²⁺ biosensor

Biosensor constructed by the ligation of parts BBa_K2486021 and BBa_K2486004.

Usage and Biology

We designed this part in order to validate our newly designed promoter PdtxR (BBa_K2486023). The biosensor was able to respond to different concentrations of iron, which suggests it could be used to detect and differentiate Fe²⁺ content of the blood.

Validation

T--USP-Brazil--Detecttion_Iron_Lactate_dtxR%281%29.png Figure 1 - Promoter activity (GFP/OD600) under different concentrations of Iron Chloride (Fe²⁺)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 843
Illegal NheI site found at 866
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1420
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 716

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2486021 short</partinfo>
-Intermediarry assembly between parts BBa_K2486021 and BBa_K2486004
+Biosensor constructed by the ligation of parts BBa_K2486021 and BBa_K2486004.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We designed this part in order to validate our newly designed promoter PdtxR (BBa_K2486023).
+The biosensor was able to respond to different concentrations of iron, which suggests it could be used to detect and differentiate Fe<sup>2+</sup> content of the blood.
+====Validation====
+https://static.igem.org/mediawiki/2017/c/cc/T--USP-Brazil--Detecttion_Iron_Lactate_dtxR%281%29.png
+Figure 1 - Promoter activity (GFP/OD600) under different concentrations of Iron Chloride (Fe<sup>2+</sup>)
 <!-- -->