Difference between revisions of "Part:BBa K2520000"
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The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. | The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. | ||
We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR. | We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR. | ||
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| + | [[File:B7-1.png|400px|thumb|center|Figure 1: Membrane-anchored single-chain antibodies. A scFv is composed of the immunoglobulin heavy and light chain variable regions linked together with a flexible peptide (normally 15 amino acids in length). For expression of a membrane-anchored scFv, a signal peptide (SP) directs the nascent chain to the lumen of the endoplasmic reticulum whereas the TM anchors the antibody in the plasma membrane. ]] | ||
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| + | ===References=== | ||
| + | Cheng, Tian‐Lu, and Steve Roffler. "Membrane‐tethered proteins for basic research, imaging, and therapy." Medicinal research reviews 28.6 (2008): 885-928. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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<partinfo>BBa_K2520000 parameters</partinfo> | <partinfo>BBa_K2520000 parameters</partinfo> | ||
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Latest revision as of 09:51, 29 October 2017
B7-1 Murine Trans Membrane Domain
The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR.
Figure 1: Membrane-anchored single-chain antibodies. A scFv is composed of the immunoglobulin heavy and light chain variable regions linked together with a flexible peptide (normally 15 amino acids in length). For expression of a membrane-anchored scFv, a signal peptide (SP) directs the nascent chain to the lumen of the endoplasmic reticulum whereas the TM anchors the antibody in the plasma membrane.
References
Cheng, Tian‐Lu, and Steve Roffler. "Membrane‐tethered proteins for basic research, imaging, and therapy." Medicinal research reviews 28.6 (2008): 885-928.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]