Difference between revisions of "Part:BBa K2201243"

Latest revision as of 19:57, 24 October 2017

Fusion protein of CFP and YFP with an amber codon in the linker

This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014] and BBa_K2020040 of the [http://2016.igem.org/Team:Aachen/Description#characterization iGEM-team Aachen 2016]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.

Important: This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 718
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1308
Illegal AgeI site found at 1420
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2201243 short</partinfo>
-This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, <b>[https://parts.igem.org/Part:BBa_K1416004 BBa_K1416004]</b>) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014] and <b>[https://parts.igem.org/Part:BBa_K2020040BBa_K2020040]</b> of the [http://2016.igem.org/Team:Aachen/Description#characterization iGEM-team Aachen 2016]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
+This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, <b>[https://parts.igem.org/Part:BBa_K1416004 BBa_K1416004]</b>) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014] and <b>[https://parts.igem.org/Part:BBa_K2020040 BBa_K2020040]</b> of the [http://2016.igem.org/Team:Aachen/Description#characterization iGEM-team Aachen 2016]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
 <b>Important:</b> This part does not contain a promoter! For the functional part and the results, look up to <b>[https://parts.igem.org/Part:BBa_K2201343 BBa_K2201343]</b>.