Difference between revisions of "Part:BBa K2273000:Design"
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<partinfo>BBa_K2273000 SequenceAndFeatures</partinfo><br>
 
<partinfo>BBa_K2273000 SequenceAndFeatures</partinfo><br>
 
===Design===
 
===Design===
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This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>srfA</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
+
This part was generated with RFC10 Suffix and Prefix. It encodes for the <i>srfA</i> promoter that is part of the ComX quorum response of <i>B. subtilis</i> and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].<br>
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===Source===
 
===Source===
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The <i>srfA</i> promoter of <i>B. subtilis</i> was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
+
The <i>srfA</i> promoter was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
 
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<table>
 
<table>
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</tr>
 
</tr>
 
</table>
 
</table>
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===References===
 
  
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Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R <i>Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.</i> Journal of Molecular Biology 362: 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/16930615 PubMed]<br><br>
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===References===
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Jan Maarten van Dijl and Michael Hecker "<i>Bacillus subtilis</i>: from soil bacterium to super-secreting cell factory" Microbial Cell Factories 12:3 (2013): 1-6. [https://www.ncbi.nlm.nih.gov/pubmed/23311580 PubMed]<br><br>
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Ling lin Fu, Zi Rong Xu, Wei Fen Li, Jiang Bing Shuai, Ping Lu, Chun Xia Hu "Protein secretion pathways in <i>Bacillus subtilis</i>: Implication for optimization heterologous protein secretion" Biotechnology Advances 25 (2007): 1-12. [https://www.ncbi.nlm.nih.gov/pubmed/16997527 PubMed]
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DOI:10.1016/j.jbiotec.2016.03.002<br>
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Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) <i>Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.</i> Journal of Molecular Biology 362: 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/?term=Substitution%20of%20the%20Native%20srfA%20Promoter%20by%20Constitutive%20Pveg%20in%20Two%20B.%20subtilis%20Strains%20and%20Evaluation%20of%20the%20Effect%20on%20Surfactin%20Production PubMed]<br><br>
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PMCID:PMC208261<br>
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Nakano MM, Xia LA, Zuber P (1991) <i>Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis.</i> J Bacteriol. 173(17):5487-93.
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DOI: 10.1093/nar/gkv1242<br>
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[https://www.ncbi.nlm.nih.gov/pubmed/?term=Transcription%20initiation%20region%20of%20the%20srfA%20operon,%20which%20is%20controlled%20by%20the%20comP-comA%20signal%20transduction%20system%20in%20Bacillus%20subtilis. PubMed]<br><br>
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DOI: 10.1099/mic.0.26003-0<br>
+
Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) <i>The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs.</i> Nucleic Acids Res.44(5):2160-72.
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DOI: 10.1002/bit.24524<br>
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[https://www.ncbi.nlm.nih.gov/pubmed/?term=The%20quorum-sensing%20regulator%20ComA%20from%20Bacillus%20subtilis%20activates%20transcription%20using%20topologically%20distinct%20DNA%20motifs PubMed]<br><br>
 +
Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br>

Latest revision as of 18:00, 24 October 2017

PsrfA: a ComA-dependent promoter of Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the srfA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The srfA promoter was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

PsrfA fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAGTTGCGGCATCCCGCAAAAAATATTG
PsrfA rev RFC10 gatcCTGCAGCGGCCGCTACTAGTACTTTATAAGCAGTGAACATG

References

Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production. Journal of Molecular Biology 362: 393-402. PubMed

Nakano MM, Xia LA, Zuber P (1991) Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis. J Bacteriol. 173(17):5487-93. PubMed

Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res.44(5):2160-72. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed