Difference between revisions of "Part:BBa K2471002"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K2471002 short</partinfo> | ||
+ | |||
+ | This BioBrick™ contains the necessary genetic circuitry to constitutively express the epsE gene, that when expressed, results in the production of the epsE enzyme. This will cause the disengagement of the flagellum from the motor proteins in the cell membrane, promoting biofilm formation and inhibiting motility. | ||
+ | |||
+ | |||
+ | A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™. | ||
+ | |||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K2471002 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K2471002 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 05:34, 24 October 2017
(T7 promoter + RBS) + (epsE) + (T1 terminator)
This BioBrick™ contains the necessary genetic circuitry to constitutively express the epsE gene, that when expressed, results in the production of the epsE enzyme. This will cause the disengagement of the flagellum from the motor proteins in the cell membrane, promoting biofilm formation and inhibiting motility.
A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 800
Illegal AgeI site found at 550 - 1000COMPATIBLE WITH RFC[1000]