Difference between revisions of "Part:BBa K2207010"

Latest revision as of 14:49, 30 October 2017

phlo Promoter

BBa_K2207010

Promoter(phlF regulated)

This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory.

It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens and related species.

In the absence of PhlF protein, this part promotes transcription.

In the presence of PhlF protein, this part inhibits transcription.

PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).

PhlF can be coded by BBa_K2207009.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 807
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2207010 short</partinfo>
-blblblblblblbl
+BBa_K2207010
+Promoter(phlF regulated)
+This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory.
+It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens
+and related species.
+In the absence of PhlF protein, this part promotes transcription.
+In the presence of PhlF protein, this part inhibits transcription.
+PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).
+PhlF can be coded by BBa_K2207009.
 <!-- Add more about the biology of this part here