Difference between revisions of "Part:BBa K2243010"
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− | The constitutive promoter J23119 is between | + | The constitutive promoter J23119 is between TP901-1 specific sites and will be inverted by the TP901-1 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1 characterization. |
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+ | We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop. | ||
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+ | <html> | ||
+ | <body> | ||
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+ | <img src="https://static.igem.org/mediawiki/2017/f/fc/Peking_flipflop_fig_5.svg" height="500" width="600"/> | ||
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+ | </body> | ||
+ | </html> | ||
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+ | Figure 1. The standard testing system used to characterize the recombinases. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 19:03, 1 November 2017
TP901-1 attB_J23119_TP901-1 attP
The constitutive promoter J23119 is between TP901-1 specific sites and will be inverted by the TP901-1 recombinase, thus leading to the direction of promoter and change of gene expression. This unit can be used as standard reporter of recombinase TP901-1 characterization.
We use this part to be the state switch of the flip-flop. See the link of the bio-flip-flop.
Figure 1. The standard testing system used to characterize the recombinases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 105
Illegal NheI site found at 128 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 188
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]