Difference between revisions of "Part:BBa K2217027"

Latest revision as of 17:15, 26 October 2017

laci + miRNA-1825 binding site synthesized fragment

Egypt-AFCM Team Improvement

[http://2017.igem.org/Team:AFCM-Egypt# Egypt-AFCM Team] tried to improve laci function at BBa_K1179062 regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at BBa_K2217027_Experience.

laci registered at (BBa_K2217012), was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2217027 short</partinfo>
+==Egypt-AFCM Team Improvement==
+[http://2017.igem.org/Team:AFCM-Egypt# Egypt-AFCM Team] tried to improve laci function at [https://parts.igem.org/Part:BBa_K1179062# BBa_K1179062] regarding inhibition of non-coding RNAs by fusing laci  the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site.  Part characterization and usage can be found at  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2217027# BBa_K2217027_Experience].
 laci registered at [https://parts.igem.org/Part:BBa_K2217012# (BBa_K2217012)], was fused to the miRNA binding site of the miRNA mir-1825 as determined computationally using circinteractome database, where we hypothesized its function as a sensor for miRNA binding.