Difference between revisions of "Part:BBa K2243005:Design"

 
 
Line 6: Line 6:
  
  
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===Design Notes===
+
===Usage===
−
N/A
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C12_Bxb1 gp35 is one of our best expression system. We added RBS C12 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).
 +
 
 +
===Biology===
 +
The Bxb1 coding sequence is the part BBa_K22430012.
 +
 
 +
===Design note===
 +
We construct this structure by Golden Gate Assembly. The C12 RBS is generate by random mutation.
 +
 
 +
===Characterize===
 +
We characterize the C12_Bxb1 gp35 by using the standard testing system, consisting of the integrase expression plasmid and the corresponding reporter plasmid(BBa_K2243006).
 +
 
  
  

Latest revision as of 14:35, 29 October 2017


C12_Bxb1 gp35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 212
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 486
    Illegal XhoI site found at 573
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1125
    Illegal NgoMIV site found at 1212
    Illegal AgeI site found at 262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1320


Usage

C12_Bxb1 gp35 is one of our best expression system. We added RBS C12 upstream the Bxb1 gp35 coding sequence (BBa_K22430012).

Biology

The Bxb1 coding sequence is the part BBa_K22430012.

Design note

We construct this structure by Golden Gate Assembly. The C12 RBS is generate by random mutation.

Characterize

We characterize the C12_Bxb1 gp35 by using the standard testing system, consisting of the integrase expression plasmid and the corresponding reporter plasmid(BBa_K2243006).



Source

Mycobacterium Phage Bxb1

References