Difference between revisions of "Part:BBa K2217024:Experience"
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part through gel electrophoresis for experimental characterization of lentiviral transfer plasmid transfection efficacy as a strong promoter enhanced by fusing CMV enhancer.
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https://static.igem.org/mediawiki/parts/3/34/AFCMCMV1.png
  
 
===Applications of BBa_K2217024===
 
===Applications of BBa_K2217024===
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Agarose gel electrophoresis of amplified Fragments of second genetic construct (crispr-based) and gibson assembly products of both genetic constructs, in which Lane [3] is characterizing BBa_K2217024 which includes Fragment 2([CMV enhancer+ CMV promoter + T7 promoter )
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Agarose gel electrophoresis of amplified Fragments of second genetic construct (non-crispr-based) and gibson assembly products of both genetic constructs, in which Lane [2] is characterizing BBa_K2217024 which characterizes ([CMV enhancer+ CMV promoter)
  
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[[File:Image7.png]]  
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[[File:Image9.png]]  
  
  

Latest revision as of 21:50, 14 October 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

[http://2018.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt 2018] provided specific characterization for this part through gel electrophoresis for experimental characterization of lentiviral transfer plasmid transfection efficacy as a strong promoter enhanced by fusing CMV enhancer.

AFCMCMV1.png

Applications of BBa_K2217024

Agarose gel electrophoresis of amplified Fragments of second genetic construct (non-crispr-based) and gibson assembly products of both genetic constructs, in which Lane [2] is characterizing BBa_K2217024 which characterizes ([CMV enhancer+ CMV promoter)

Image9.png


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