Difference between revisions of "Part:BBa K2368005"
 
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
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<partinfo>BBa_K2368005 short</partinfo>
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<p style="text-align: center"><partinfo>BBa_K2368005 short</partinfo></p>
  
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<P><i>P<sub>fus</sub></i> is an induced promoter from <i>Saccharomyces cerevisiae</i>. In the endogenous MAPK pathway in <i>S.cerevisiae</i>, when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. </P>
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<P><i>P<sub>fus</sub></i> is an induced promoter from <i>Saccharomyces cerevisiae</i>. In the endogenous MAPK pathway in <i>Saccharomyces cerevisiae</i>, when Ste2 binding with α pheromone, Ste12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. </P>
 
<P>However, the sequence of <i>P<sub>fus</sub></i> contains <i>Pst1</i> restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
 
<P>However, the sequence of <i>P<sub>fus</sub></i> contains <i>Pst1</i> restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
 
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[[File:T_BIT-China_2017part_K2368005.jpeg|center|300px|标题 ‎]]
 
[[File:T_BIT-China_2017part_K2368005.jpeg|center|300px|标题 ‎]]
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<p style="text-align: center">fig.1 The sequencing result of <i>P<sub>fus</sub></i></p>
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<p style="text-align: center">Fig.1 The sequencing result of <i>P<sub>fus</sub></i></p>
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<p>This part is an improvement of [https://parts.igem.org/Part:BBa_K1072023 BBa_K1072023] of 2013 SCUT team.</p>
  
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<P>This part is an improvement of BBa_K775004 of 2013 SCUT.</P>
 
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 18:04, 26 October 2017


Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in Saccharomyces cerevisiae, when Ste2 binding with α pheromone, Ste12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

标题 ‎

Fig.1 The sequencing result of Pfus

This part is an improvement of BBa_K1072023 of 2013 SCUT team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]