Difference between revisions of "Part:BBa K2267001"
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<partinfo>BBa_K2267001 short</partinfo> | <partinfo>BBa_K2267001 short</partinfo> | ||
− | + | <h2>Explained </h2> | |
− | GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.[[File:T--TUST China-- | + | GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH. |
− | + | <br> | |
+ | <br> | ||
+ | Gene sequence: https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=511641&to=512573[[File:T--TUST China--parts GlsA.gif]] | ||
+ | <h2>Expressed</h2> | ||
+ | [[File:T--TUST China--parts GlsA pcr.png]] | ||
+ | <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 15:35, 24 October 2017
A gene encodes glutaminase 1;Catalyze a reaction converting glutamine to glutamate and releasing the
Explained
GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.
Gene sequence: https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=511641&to=512573
Expressed
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 967
Illegal SpeI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 967
Illegal SpeI site found at 2
Illegal NotI site found at 9
Illegal NotI site found at 959 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 967
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 967
Illegal SpeI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 967
Illegal SpeI site found at 2 - 1000COMPATIBLE WITH RFC[1000]