Difference between revisions of "Part:BBa K2247004"

 
 
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HXT5 is one of the glucose transporters of S. cerevisiae
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High-affinity glucose transporter 5 (HXT5) is a member of the HXT transmembrane glucose transporter family of S. cerevisiae. HXT5 is localized to the plasma membrane, and has glucose, fructose, mannose and pentose transmembrane transporter activity.
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===Usage and Biology===
 
===Usage and Biology===
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HXT5 is a high-affinity glucose transporter in S. cerevisiae, similar to HXT2 ([https://parts.igem.org/Part:BBa_K2247002 BBa_K2247002]).
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===Characterization===
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[[File:Constitutive HXT on glucose medium.jpeg|500px]]
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=====Figure 1. The growth of HXT-expressing yeast cells on glucose-only medium.=====
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This strain of yeast (JDYY001) is knockout of all Hexose Transporter (HXT)-related genes, and therefore cannot use glucose as a carbon source. The yeast cells were transformed with plasmids constitutively expressing HXT2, or HXT5, or empty vector as a negative control, and plated onto the medium where glucose is the only carbon source. Serial dilution clearly showed the growth of yeasts. Therefore, HXT2 or HXT5 expression can effectively allow JDYY001 grow on glucose-only medium.
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Latest revision as of 17:53, 31 October 2017


HXT5 glucose transpoter from S. cerevisiae

High-affinity glucose transporter 5 (HXT5) is a member of the HXT transmembrane glucose transporter family of S. cerevisiae. HXT5 is localized to the plasma membrane, and has glucose, fructose, mannose and pentose transmembrane transporter activity.


Usage and Biology

HXT5 is a high-affinity glucose transporter in S. cerevisiae, similar to HXT2 (BBa_K2247002).


Characterization

Constitutive HXT on glucose medium.jpeg


Figure 1. The growth of HXT-expressing yeast cells on glucose-only medium.

This strain of yeast (JDYY001) is knockout of all Hexose Transporter (HXT)-related genes, and therefore cannot use glucose as a carbon source. The yeast cells were transformed with plasmids constitutively expressing HXT2, or HXT5, or empty vector as a negative control, and plated onto the medium where glucose is the only carbon source. Serial dilution clearly showed the growth of yeasts. Therefore, HXT2 or HXT5 expression can effectively allow JDYY001 grow on glucose-only medium.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1740
    Illegal SapI.rc site found at 193