Difference between revisions of "Part:BBa K2314024"
 
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yEcitrine is one of the improved variants of YFP. It has been widely used in yeast and has a good performance than unoptimized YFP.
 
yEcitrine is one of the improved variants of YFP. It has been widely used in yeast and has a good performance than unoptimized YFP.
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The  excitation/emission pair (in nm) is 502/532. Ref: Sheff, Mark A., and Kurt S. Thorn. "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae." Yeast 21.8 (2004): 661-670.
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The  excitation/emission pair (in nm) is 502/532.  
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Reference:
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[1] Sheff, Mark A., and Kurt S. Thorn. "Optimized cassettes for fluorescent protein tagging in <i>Saccharomyces cerevisiae</i>." Yeast 21.8 (2004): 661-670.
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The structure of yEcitrine.
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The structure of YEc
 
 
https://static.igem.org/mediawiki/parts/8/84/T--OUC-China--INPmSA-yEC.gif  
 
https://static.igem.org/mediawiki/parts/8/84/T--OUC-China--INPmSA-yEC.gif  
  

Latest revision as of 16:28, 1 November 2017


yEcitrine is one of the improved variants of YFP. It has been widely used in yeast.

yEcitrine is one of the improved variants of YFP. It has been widely used in yeast and has a good performance than unoptimized YFP. The excitation/emission pair (in nm) is 502/532.

Reference: [1] Sheff, Mark A., and Kurt S. Thorn. "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae." Yeast 21.8 (2004): 661-670.

The structure of yEcitrine.


T--OUC-China--INPmSA-yEC.gif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644