Difference between revisions of "Part:BBa K2332017:Design"

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===Design Notes===
 
===Design Notes===
−
We removed the stop codong in GFP and placed the SpyTag sequence after a linker sequence.
+
We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence.
  
 
===Source===
 
===Source===
  
−
https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence
+
SpyTag sequence was obtained from [https://parts.igem.org/Part:BBa_K1159201 BBa_K1159201] and GFP from:
−
https://parts.igem.org/Part:BBa_E0040 was for the GFP
+
[https://parts.igem.org/Part:BBa_E0040 BBa_E0040]. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
  
 
===References===
 
===References===
 +
Zakeri B, Fierer J, Celik E, Chittock E, Schwarz-Linek U, Moy V et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proceedings of the National Academy of Sciences. 2012;109(12):E690-E697.

Latest revision as of 21:58, 22 October 2017


GFP-SpyTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence.

Source

SpyTag sequence was obtained from BBa_K1159201 and GFP from: BBa_E0040. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References

Zakeri B, Fierer J, Celik E, Chittock E, Schwarz-Linek U, Moy V et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proceedings of the National Academy of Sciences. 2012;109(12):E690-E697.