Difference between revisions of "Part:BBa K2486005:Experience"
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===Applications of BBa_K2486005=== | ===Applications of BBa_K2486005=== | ||
| + | This part was used in our project as an exogenous iron sensor for E. coli and P. agglomerans. The repressor should detect an increase in Fe2+ levels in the midgut of anophelines fed with blood and regulate the expression of antiparasitic peptides (against Plasmodium spp., as a proof of concept). As dtxR is a repressor in the presence of Fe2+ we have coupled it to a logic inverter (TetR) which ultimately regulates the expression of our system in combination with a lactate detector (LldR). We have selected this regulator instead of the endogenous Fur (ferric uptake regulator) in order to avoid metabolic interferences as there are no DNA binding motifs for dtxR in the genome of our chassis (Boyd, 1998). | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 23:21, 24 October 2017
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2486005
This part was used in our project as an exogenous iron sensor for E. coli and P. agglomerans. The repressor should detect an increase in Fe2+ levels in the midgut of anophelines fed with blood and regulate the expression of antiparasitic peptides (against Plasmodium spp., as a proof of concept). As dtxR is a repressor in the presence of Fe2+ we have coupled it to a logic inverter (TetR) which ultimately regulates the expression of our system in combination with a lactate detector (LldR). We have selected this regulator instead of the endogenous Fur (ferric uptake regulator) in order to avoid metabolic interferences as there are no DNA binding motifs for dtxR in the genome of our chassis (Boyd, 1998).
User Reviews
UNIQ66670331826b875b-partinfo-00000000-QINU UNIQ66670331826b875b-partinfo-00000001-QINU