Difference between revisions of "Part:BBa K2505006"
 
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<partinfo>BBa_K2505006 short</partinfo>
 
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<span style="margin-left: 25px;">This gene is a promoter from <i>E.coli</i> and activated by some stress like osmotic pressure or low temperature that involved in synthesis of the colanic acid capsular polysaccharide in <i>E.coli</i>. The genes(<i>cps</i>) are transcriptionally regulated by numerous proteins: RcsB, RcsC and AHK4.This promoter includes native rbs: ribosome binding site.
 
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This is a promoter derived from <i>E. coli</i> and acativated through histidine-to-aspartate phosphorelay. A His-to-Asp phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. One of the His-to-Asp phosphorelay systems in <i>E. coli</i> is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, and RcsB, a response regulator. This system is activated after stress exposure such as osmolality shock; <i>cps</i> operon promoter (which controls the production of polysaccharides) is induced through the RcsC→RcsD→RscB→<i>cps</i> pathway.
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==Characterization==
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==Result==
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NOT DATA
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==Materials & Methods==
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NOT DATA
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==Reference==
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Suzuki, T., Miwa, K., Ishikawa, K., Yamada, H., Aiba, H. and Mizuno, T. (2001) The Arabidopsis Sensor His-kinase, AHK4, Can Respond to Cytokinins. Plant Cell Physiol. 42: 107-113.
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Spíchal, L., Rakova, N.Y., Riefler, M., Mizuno, T., Romanov, G.A.,Strnad, M. and Schmülling, T. (2004) Two Cytokinin Receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, Differ in their Ligand Specifity in a Bacterial Assay. Plant Cell Physiol. 45: 1299-1305.

Latest revision as of 18:32, 1 November 2017

Pcps

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This is a promoter derived from E. coli and acativated through histidine-to-aspartate phosphorelay. A His-to-Asp phosphorelay system is one of the most important signal transduction systems for prokaryotes to respond to environmental stimuli. One of the His-to-Asp phosphorelay systems in E. coli is composed of three components: RcsC, a histidine kinase, RcsD, a histidine-containing phosphotransmitter, and RcsB, a response regulator. This system is activated after stress exposure such as osmolality shock; cps operon promoter (which controls the production of polysaccharides) is induced through the RcsC→RcsD→RscB→cps pathway.


Characterization

Result

NOT DATA

Materials & Methods

NOT DATA

Reference

Suzuki, T., Miwa, K., Ishikawa, K., Yamada, H., Aiba, H. and Mizuno, T. (2001) The Arabidopsis Sensor His-kinase, AHK4, Can Respond to Cytokinins. Plant Cell Physiol. 42: 107-113.

Spíchal, L., Rakova, N.Y., Riefler, M., Mizuno, T., Romanov, G.A.,Strnad, M. and Schmülling, T. (2004) Two Cytokinin Receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, Differ in their Ligand Specifity in a Bacterial Assay. Plant Cell Physiol. 45: 1299-1305.