Difference between revisions of "Part:BBa K2294000"

 
 
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<partinfo>BBa_K2294000 short</partinfo>
 
<partinfo>BBa_K2294000 short</partinfo>
  
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This part is a fusion between the SV40 nuclear localization signal and a thermostable version of the Saccharomyces cerevisiae site-specific recombinase FLP. Recombination occurs at FRT sequences.See https://en.wikipedia.org/wiki/FLP-FRT_recombination and https://en.wikipedia.org/wiki/Nuclear_localization_sequence for more information.  
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This part is an improvement of the original part BBa_K313002 (<html><a href="https://parts.igem.org/Part:BBa_K313002" target="_blank">BBa_K2294000</a></html>
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)
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This part is a fusion of<br>
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•  FLPe, a thermostable version of the <i>S. cerevisiae</i> FRT site specific recombinase FLP and<br>
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•  NLS, a SV40 nuclear localization sequence. <br>
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For more information see https://en.wikipedia.org/wiki/FLP-FRT_recombination and https://en.wikipedia.org/wiki/Nuclear_localization_sequence.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:13, 30 October 2017


FLPe + NLS

This part is an improvement of the original part BBa_K313002 (BBa_K2294000 )

This part is a fusion of
• FLPe, a thermostable version of the S. cerevisiae FRT site specific recombinase FLP and
• NLS, a SV40 nuclear localization sequence.

For more information see https://en.wikipedia.org/wiki/FLP-FRT_recombination and https://en.wikipedia.org/wiki/Nuclear_localization_sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]