Difference between revisions of "Part:BBa K2371002:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Unsure...
 
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===Source===
 
===Source===
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Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.
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===References===
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1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216.
  
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T7 polymerase from bacteria BL21(DE3)
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2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.
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===References===
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Latest revision as of 14:41, 31 October 2017


N-T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence of T7 polymerase is obtained from Eco.li BL21(DE3), whose genome is genetically modified to contain the coding sequence for T7 polymerase.

References

1.Yihao,Z.et al.,2017.Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains. ACS Synth. Biol., 2017, 6 (2), pp 211–216.

2.Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.