Difference between revisions of "Part:BBa K2440025"

 
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<partinfo>BBa_K2440025 short</partinfo>
 
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It is the target sequence of miR165a, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
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===Usage and Biology===
 
===Usage and Biology===
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MiRNA locker assembled by using this modularized DNA part was able to bind miR165a in an Ago2 dependent manner, that is, knockdown of miR165a was achieved by transfecting cells with miRNA locker.
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Aly-miR165a-5p is a novel miRNA that was firstly found in Arabidopsis lyrata<sup>1</sup>. And its function is still unknown.
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===Sequence and Features===
  
 
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<partinfo>BBa_K2440025 parameters</partinfo>
 
<partinfo>BBa_K2440025 parameters</partinfo>
 
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==Experimental Validation==
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This part is sequenced as correct after construction.
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==Reference==
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1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010).

Latest revision as of 01:10, 1 November 2017


miR165a target sequence

It is the target sequence of miR165a, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR165a in an Ago2 dependent manner, that is, knockdown of miR165a was achieved by transfecting cells with miRNA locker.

Aly-miR165a-5p is a novel miRNA that was firstly found in Arabidopsis lyrata1. And its function is still unknown.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Ma Z, Coruh C, Axtell MJ Plant Cell. 22:1090-1103(2010).