Difference between revisions of "Part:BBa K2440014"
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<partinfo>BBa_K2440014 short</partinfo> | <partinfo>BBa_K2440014 short</partinfo> | ||
− | + | It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker. | ||
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+ | Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.<sup>1</sup> | ||
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+ | MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase.<sup>2</sup> | ||
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+ | ===Sequence and Features=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K2440014 parameters</partinfo> | <partinfo>BBa_K2440014 parameters</partinfo> | ||
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+ | ==Experimental Validation== | ||
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+ | This part is sequenced as correct after construction. | ||
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+ | ==Reference== | ||
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+ | 1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003). | ||
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+ | 2.MicroRNA-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase/Wnt signaling pathway.Xu Z, Li C, Qu H, Li H, Gu Q, Xu J. |
Latest revision as of 01:36, 1 November 2017
miR-195 target sequence
It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.
Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.1
MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase.2
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2.MicroRNA-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase/Wnt signaling pathway.Xu Z, Li C, Qu H, Li H, Gu Q, Xu J.