Difference between revisions of "Part:BBa K2407103:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
to check if the transformation is successful.
+
This part is to check if the transformation is successful.
−
 
+
And this part also produces L-Isoleucine to live on.
  
  
Line 16: Line 16:
  
 
===References===
 
===References===
 +
Hicks WM, Kim M, Haber JE Increased mutagenesis and unique mutation signature associated with mitotic gene conversion. 10.1126/science.1191125.

Latest revision as of 04:34, 26 October 2017


Leu-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2049
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is to check if the transformation is successful. And this part also produces L-Isoleucine to live on.


Source

A plasmid from YJ lab

References

Hicks WM, Kim M, Haber JE Increased mutagenesis and unique mutation signature associated with mitotic gene conversion. 10.1126/science.1191125.