Difference between revisions of "Part:BBa K2407101:Design"
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
This sequence is modified for use in yeast or other AT-rich fungi.
 
This sequence is modified for use in yeast or other AT-rich fungi.
−
This part is used to express the transcription factor, Z4EV.  
+
This part is used to express the transcription factor, Z4EV. Z4EV are fast-acting and achieve a graded output response over a range of inducer concentrations.  
 
And the gene HO need to be promoted the transcription of Z4EV.
 
And the gene HO need to be promoted the transcription of Z4EV.
  
Line 17: Line 17:
  
 
===References===
 
===References===
 +
R. Scott McIsaac,* Benjamin L. Oakes, Xin Wang, Krysta A. Dummit, David Botstein, and Marcus B. Noyes Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast  10.1093/nar/gks1313

Latest revision as of 09:07, 26 October 2017


express the transcription factor, Z4EV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 1162
    Illegal EcoRI site found at 1959
    Illegal EcoRI site found at 1991
    Illegal EcoRI site found at 2511
    Illegal PstI site found at 1447
    Illegal PstI site found at 1561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 1162
    Illegal EcoRI site found at 1959
    Illegal EcoRI site found at 1991
    Illegal EcoRI site found at 2511
    Illegal PstI site found at 1447
    Illegal PstI site found at 1561
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 1162
    Illegal EcoRI site found at 1959
    Illegal EcoRI site found at 1991
    Illegal EcoRI site found at 2511
    Illegal BglII site found at 1527
    Illegal BglII site found at 1809
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 1162
    Illegal EcoRI site found at 1959
    Illegal EcoRI site found at 1991
    Illegal EcoRI site found at 2511
    Illegal PstI site found at 1447
    Illegal PstI site found at 1561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 1162
    Illegal EcoRI site found at 1959
    Illegal EcoRI site found at 1991
    Illegal EcoRI site found at 2511
    Illegal PstI site found at 1447
    Illegal PstI site found at 1561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1073
    Illegal BsaI.rc site found at 840
    Illegal BsaI.rc site found at 2566


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. This part is used to express the transcription factor, Z4EV. Z4EV are fast-acting and achieve a graded output response over a range of inducer concentrations. And the gene HO need to be promoted the transcription of Z4EV.


Source

We use overlap PCR to connect a piece of Can-A, TEF1 with Z4EV.

References

R. Scott McIsaac,* Benjamin L. Oakes, Xin Wang, Krysta A. Dummit, David Botstein, and Marcus B. Noyes Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast 10.1093/nar/gks1313