Difference between revisions of "Part:BBa K2407100:Design"

(→‎Design Notes)
 
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===References===
 
===References===
 +
Hanson SJ, Wolfe KH .An Evolutionary Perspective on Yeast Mating-Type Switching.10.1534/genetics.117.202036.

Latest revision as of 04:20, 26 October 2017


a composite part to control the expression of HO gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
    Illegal BglII site found at 902
    Illegal BglII site found at 1185
    Illegal BglII site found at 1869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742 It is a composite part to control the expression of HO gene. What's more, it triggers the transformation of their mating type.

Source

We connect the segments(Gal-HO-CYC1) by T4 DNA Ligase.


References

Hanson SJ, Wolfe KH .An Evolutionary Perspective on Yeast Mating-Type Switching.10.1534/genetics.117.202036.