Difference between revisions of "Part:BBa I729004"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I729004 short</partinfo>
 
<partinfo>BBa_I729004 short</partinfo>
  
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This AHL receiver contains mutant LuxR.
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This AHL receiver contains mutant LuxR.(I45F)
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it can express GFP more at AHL 10nM compared with wild type.
  
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it can express GFP 0.1x AHL conc. compared wild type.
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(Ref. Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:25, 26 October 2007

Sensitive AHL Receiver

This AHL receiver contains mutant LuxR.(I45F)

it can express GFP more at AHL 10nM compared with wild type.

(Ref. Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 979
    Illegal BsaI.rc site found at 1707