Difference between revisions of "Part:BBa K2446010:Design"
(→References) |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | This part is assembled with two BBa_K2446003 by Biobrick assembly in pSB1C3. | |
===References=== | ===References=== | ||
| + | Morsut, L. et al. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016). | ||
Latest revision as of 02:23, 29 October 2017
4_ZFHD1 binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
Source
This part is assembled with two BBa_K2446003 by Biobrick assembly in pSB1C3.
References
Morsut, L. et al. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016).