Difference between revisions of "Part:BBa K2446009:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells. | |
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===Source=== | ===Source=== | ||
| − | + | This part was assembled with two BBa_K2446002 by biobrick assembly in pSB1C3. | |
===References=== | ===References=== | ||
| + | Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012). | ||
Latest revision as of 02:17, 29 October 2017
4_ZF54-8/43-8 binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of Zinc DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
Source
This part was assembled with two BBa_K2446002 by biobrick assembly in pSB1C3.
References
Lohmueller, J. J., Armel, T. Z. & Silver, P. A. A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012).