Difference between revisions of "Part:BBa K2446004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of PIP DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | IDT (gBlocks) | |
===References=== | ===References=== | ||
| + | Fussenegger, M. et al. Streptogramin-based gene regulation systems for mammalian cells. Nature biotechnology 18, 1203--1208 (2000). | ||
Latest revision as of 18:32, 28 October 2017
2_PIP binding site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used as a operator of our synthetic promoters. This operator will be inserted around the promoter of minCMV or Sv40 and bound by our synthetic transcription factor, which is made of PIP DNA binding domain fusing to VP64 or KRAB. In this way, we can create a group of activating or silencing synthetic promoter-transcription factor pairs in mammalian cells.
Source
IDT (gBlocks)
References
Fussenegger, M. et al. Streptogramin-based gene regulation systems for mammalian cells. Nature biotechnology 18, 1203--1208 (2000).