Difference between revisions of "Part:BBa K2201026"

 
 
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<partinfo>BBa_K2201026 short</partinfo>
 
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Plasmid for the retention of unnatural base pairs
 
  
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===Usage and Biology===
 
===Usage and Biology===
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This part was used as a backbone for the integration of fragments containing unnatural base pairs. The insertion site is located upstream of mRFP, providing an easy screening method. For more on the retention and preservation system, please visit our <a href="http://2017.igem.org/Team:Bielefeld-CeBiTec/Project/unnatural_base_pair/preservation_system">wiki!</a>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2201026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2201026 SequenceAndFeatures</partinfo>

Latest revision as of 13:24, 1 November 2017


Plasmid for the integration of unnatural base pairs


Usage and Biology

This part was used as a backbone for the integration of fragments containing unnatural base pairs. The insertion site is located upstream of mRFP, providing an easy screening method. For more on the retention and preservation system, please visit our wiki!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]