Difference between revisions of "Part:BBa K2209000"
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The regulating protein coding by R gene of aniline dioxygenase can bind to the promoter which inhibiting the transcription and expression of aniline dioxygenase. The key sequences of the promoter could be random mutated which interfering the binding of regulating protein and stimulating transcription and expression of the gene. Two base of the nucleotide in promoter were mutated in this study. | The regulating protein coding by R gene of aniline dioxygenase can bind to the promoter which inhibiting the transcription and expression of aniline dioxygenase. The key sequences of the promoter could be random mutated which interfering the binding of regulating protein and stimulating transcription and expression of the gene. Two base of the nucleotide in promoter were mutated in this study. | ||
| − | + | === Characterization of part BBa_K2209000 === | |
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It is expected that aniline dioxygenase from environment samples could constitutive expression by the modification. Experiment result showed that it is achieved as follows, the gene was expressed whether it is induced. | It is expected that aniline dioxygenase from environment samples could constitutive expression by the modification. Experiment result showed that it is achieved as follows, the gene was expressed whether it is induced. | ||
| + | [[Image:pic 1.png|500px|left|thumb|]] | ||
Latest revision as of 07:16, 20 October 2017
Modified promoter of aniline dioxygenase
The regulating protein coding by R gene of aniline dioxygenase can bind to the promoter which inhibiting the transcription and expression of aniline dioxygenase. The key sequences of the promoter could be random mutated which interfering the binding of regulating protein and stimulating transcription and expression of the gene. Two base of the nucleotide in promoter were mutated in this study.
Characterization of part BBa_K2209000
It is expected that aniline dioxygenase from environment samples could constitutive expression by the modification. Experiment result showed that it is achieved as follows, the gene was expressed whether it is induced.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]